Intracellular Restriction Of A Productive Noncytop

J Virol. 2008 January; 82(1): 451460. Published online 2007 October 24. doi: 10.1128/JVI.01251-07.PMCID: PMC2224397
Copyright © 2008, American confederacy for MicrobiologyIntracellular Restriction of a Productive Noncytopathic Coronavirus Infection Olga Slobodskaya, Alexander Laarman,# and Willy J. M. Spaan*molecular(a) Virology Laboratory, Department of medical checkup Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. loge 9600, 2300 RC Leiden, The Netherlands*Corresponding author. Mailing address: Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, LUMC E4-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31715261652. Fax: 31715261667. E-mail: w.j.m.spaan@lumc.nl # symbolize address: Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.Received June 8, 2007; original October 13, 2007.· OTHER SECTIONS? OABSTRACTOMATERIALS AND METHODSORESULTSODISCUSSIONOREFERENCESABSTRACTVirus infection in vitro can all result in a cytopathic effect (CPE) or survive without visible changes in infected cells (noncytopathic infection). We are interested in understanding the mechanisms controlling the impact of coronavirus infection on emcee cells.



To this end, we compared a productive, noncytopathic infection of murine hepatitis virus (MHV) strain A59 in the fibroblastlike cell line NIH 3T3 with cytopathic MHV infections. Infected NIH 3T3 cells could be cultured for up to 4 weeks without apparent CPE and yet produce virus at 107 to 108 PFU/ml. Using flow cytometry, we demonst treasured that NIH 3T3 cells expressed as frequently MHV receptor CEACAM1 as other cell lines which die from MHV infection. In contrast, using quantitative reverse transcription-PCR and metabolic labeling of ribonucleic acid, we found that the pose of viral RNA amplification in NIH 3T3 cells was lower than the rate in cells in which MHV induces a CPE. The rate of cellular RNA synthesis in...If you want to get a full essay, rove it on our website: Ordercustompaper.com



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